A high-throughput standing surface acoustic wave (SSAW)-based cell sorter
Identifieur interne : 000153 ( Main/Exploration ); précédent : 000152; suivant : 000154A high-throughput standing surface acoustic wave (SSAW)-based cell sorter
Auteurs : Liqiang Ren [États-Unis] ; Yuchao Chen [États-Unis] ; Peng Li [États-Unis] ; Zhangming Mao [États-Unis] ; Po-Hsun Huang [États-Unis] ; Joseph Rufo [États-Unis] ; Feng Guo [États-Unis] ; Lin Wang [États-Unis] ; J. Philip Mccoy [États-Unis] ; Stewart J. Levine [États-Unis] ; Tony Jun Huang [États-Unis]Source :
- Lab on a chip [ 1473-0197 ] ; 2015.
Abstract
Acoustic-based fluorescence activated cell sorters (FACS) have drawn increased attention in recent years due to their versatility, high biocompatibility, high controllability, and simple design. However, the sorting throughput for existing acoustic cell sorters is far from optimum for practical applications. Here we report a high-throughput cell sorting method based on standing surface acoustic waves (SSAWs). We utilized a pair of focused interdigital transducers (FIDTs) to generate SSAW with high resolution and high energy efficiency. As a result, the sorting throughput is improved significantly from conventional acoustic-based cell sorting methods. We demonstrated the successful sorting of 10 μm polystyrene particles with a minimum actuation time of 72 μs, which translates to a potential sorting rate of more than 13,800 events/s. Without using a cell-detection unit, we were able to demonstrate an actual sorting throughput of 3,300 events/s. Our sorting method can be conveniently integrated with upstream detection units, and it represents an important development towards a functional acoustic-based FACS system.
Url:
DOI: 10.1039/c5lc00706b
PubMed: 26289231
PubMed Central: 4641751
Affiliations:
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Yuchao" uniqKey="Chen Y" first="Yuchao" last="Chen">Yuchao Chen</name><affiliation wicri:level="4"><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Li, Peng" sort="Li, Peng" uniqKey="Li P" first="Peng" last="Li">Peng Li</name><affiliation wicri:level="4"><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Mao, Zhangming" sort="Mao, Zhangming" uniqKey="Mao Z" first="Zhangming" last="Mao">Zhangming Mao</name><affiliation wicri:level="4"><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Huang, Po Hsun" sort="Huang, Po Hsun" uniqKey="Huang P" first="Po-Hsun" last="Huang">Po-Hsun Huang</name><affiliation wicri:level="4"><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Rufo, Joseph" sort="Rufo, Joseph" uniqKey="Rufo J" first="Joseph" last="Rufo">Joseph Rufo</name><affiliation wicri:level="4"><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name><affiliation wicri:level="4"><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><affiliation wicri:level="1"><nlm:aff id="A2">Ascent Bio-Nano Technologies, Inc., State College, PA, 16802, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Ascent Bio-Nano Technologies, Inc., State College, PA, 16802</wicri:regionArea><wicri:noRegion>16802</wicri:noRegion></affiliation></author><author><name sortKey="Mccoy, J Philip" sort="Mccoy, J Philip" uniqKey="Mccoy J" first="J. Philip" last="Mccoy">J. Philip Mccoy</name><affiliation wicri:level="2"><nlm:aff id="A3">National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Levine, Stewart J" sort="Levine, Stewart J" uniqKey="Levine S" first="Stewart J." last="Levine">Stewart J. Levine</name><affiliation wicri:level="2"><nlm:aff id="A3">National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Heart, Lung, and Blood Institute (NHLBI), NIH, Bethesda, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Huang, Tony Jun" sort="Huang, Tony Jun" uniqKey="Huang T" first="Tony Jun" last="Huang">Tony Jun Huang</name><affiliation wicri:level="4"><nlm:aff id="A1">Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author></analytic><series><title level="j">Lab on a chip</title><idno type="ISSN">1473-0197</idno><idno type="eISSN">1473-0189</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Acoustic-based fluorescence activated cell sorters (FACS) have drawn increased attention in recent years due to their versatility, high biocompatibility, high controllability, and simple design. However, the sorting throughput for existing acoustic cell sorters is far from optimum for practical applications. Here we report a high-throughput cell sorting method based on standing surface acoustic waves (SSAWs). We utilized a pair of focused interdigital transducers (FIDTs) to generate SSAW with high resolution and high energy efficiency. As a result, the sorting throughput is improved significantly from conventional acoustic-based cell sorting methods. We demonstrated the successful sorting of 10 μm polystyrene particles with a minimum actuation time of 72 μs, which translates to a potential sorting rate of more than 13,800 events/s. Without using a cell-detection unit, we were able to demonstrate an actual sorting throughput of 3,300 events/s. Our sorting method can be conveniently integrated with upstream detection units, and it represents an important development towards a functional acoustic-based FACS system.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li><li>Pennsylvanie</li></region><settlement><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Ren, Liqiang" sort="Ren, Liqiang" uniqKey="Ren L" first="Liqiang" last="Ren">Liqiang Ren</name></region><name sortKey="Chen, Yuchao" sort="Chen, Yuchao" uniqKey="Chen Y" first="Yuchao" last="Chen">Yuchao Chen</name><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name><name sortKey="Huang, Po Hsun" sort="Huang, Po Hsun" uniqKey="Huang P" first="Po-Hsun" last="Huang">Po-Hsun Huang</name><name sortKey="Huang, Tony Jun" sort="Huang, Tony Jun" uniqKey="Huang T" first="Tony Jun" last="Huang">Tony Jun Huang</name><name sortKey="Levine, Stewart J" sort="Levine, Stewart J" uniqKey="Levine S" first="Stewart J." last="Levine">Stewart J. Levine</name><name sortKey="Li, Peng" sort="Li, Peng" uniqKey="Li P" first="Peng" last="Li">Peng Li</name><name sortKey="Mao, Zhangming" sort="Mao, Zhangming" uniqKey="Mao Z" first="Zhangming" last="Mao">Zhangming Mao</name><name sortKey="Mccoy, J Philip" sort="Mccoy, J Philip" uniqKey="Mccoy J" first="J. Philip" last="Mccoy">J. Philip Mccoy</name><name sortKey="Rufo, Joseph" sort="Rufo, Joseph" uniqKey="Rufo J" first="Joseph" last="Rufo">Joseph Rufo</name><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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